Expression of αGal epitopes in the blood of reconstituted mice.

Comparison of αGal epitope expression on blood cells from a wild-type GT⁺ mouse (A), a GT⁰ mouse reconstituted with NEO^r-transduced bone marrow (B), or a GT⁰ mouse reconstituted with LGTA7-transduced bone marrow (C). Blood samples from reconstituted mice were analyzed 5 weeks after bone marrow transplantation. Shown is the pattern of CD19 (B-cell lineage) versus IB4 lectin (specific for αGal) staining gated on the total lymphocyte population. Expression of αGal epitopes was analyzed on CD19-positive cells because B-lineage cells, even in wild-type mice, express relatively high levels of αGal epitopes (J.B. and J.I., personal observation). Samples shown are from representative mice.