Cleavage of ZPI during coagulation of plasma.

ZPI in plasma and serum samples (see “Materials and methods”) was immunoadsorbed with rabbit polyclonal anti-ZPI antibodies bound to Affigel and detected following SDS-PAGE by Western blotting with monoclonal anti-ZPI antibody (MC4249.2). (A) Lane 1: normal plasma; lane 2: normal plasma treated with kaolin, phospholipids, and Ca⁺⁺; lane 3: normal plasma treated with kaolin alone; lane 4: factor XI–deficient plasma treated with kaolin alone; lane 5: normal plasma treated with tissue thromboplastin and Ca⁺⁺; lane 6: factor XI–deficient plasma treated with tissue thromboplastin and Ca⁺⁺. (B) Lane 1: factor X–deficient plasma; lane 2: factor X–deficient plasma treated with kaolin, phospholipids, and Ca⁺⁺; lane 3: factor X–deficient plasma treated with tissue thromboplastin and Ca⁺⁺; lane 4: barium adsorbed plasma (BAP) treated with XCP, phospholipids, and Ca⁺⁺; lane 5: BAP supplemented with factor X treated with XCP, phospholipids, and Ca⁺⁺; lane 6: BAP supplemented with factor X and PZ treated with XCP, phospholipids, and Ca⁺⁺.