Specific lysis of RESEEESVSL-loaded female EBV-LCL target cells by HLA-B60 HY CTL isolated from a patient with MDS.
IFNγ-secreting (IFNγ+) T cells and IFNγ-nonsecreting (IFNγ−) T cells were isolated from the PBMC of the patient with MDS after stimulation with peptide RESEEESVSL and were tested for specific reactivity. EBV-LCL cells were 51Cr-labeled for 1 hour. After washing, the female EBV cells were incubated for 1 hour with either the HYB60 peptide RESEEESVSL or the HYA2 peptide FIDSYICQV. IFNγ+ and IFNγ− T cells were added at the indicated effector-to-target ratios, and 51Cr release was measured after 4 hours. Auto EBV cells were autologous HLA-B60 and HLA-A2+ EBV cells derived from the female patient with MDS. Allo EBV cells were allogeneic HLA-B60 and HLA-A2+ female EBV cells.
