Polymorphisms in H-Y epitope differentially contributed to HLA-B60 recognition.

Female HLA-B60+ EBV-LCL cells were ⁵¹Cr-labeled for 1 hour. After washing, the cells were incubated for 1 hour with indicated peptides at various concentrations. HLA-B60 HY CTL was added at an effector-to-target ratio of 10:1, and ⁵¹Cr release was measured after 4 hours. (A) Lysis of female EBV-LCL target cells by HLA-B60 HY CTL after loading with the 10-residue UTY-derived peptide RESEEESVSL or 3 peptides each containing 1 UTX-homologue amino acid on position 1, 6, or 8. (B) Lysis of female EBV-LCL target cells by HLA-B60 HY CTL after loading with the 10-residue UTY-derived peptide RESEEESVSL or 3 peptides each containing 2 UTX-homologue amino acids.