Specific lysis of RESEEESVSL-loaded female EBV-LCL target cells by the HLA-B60 HY CTL.

Female HLA-B60+ EBV-LCL cells were ⁵¹Cr-labeled for 1 hour. After washing, the cells were incubated for 1 hour with UTY-derived peptides at various concentrations. HLA-B60 HY CTL was added at an effector-to-target ratio of 10:1, and ⁵¹Cr release was measured after 4 hours. (A) Lysis of female EBV-LCL target cells by the HLA-B60 HY CTL after loading with different peptides encoded by minigene 79-120. (B) Lysis of female EBV-LCL target cells by the HLA-B60 HY CTL after loading with the 10-residue H-Y peptide RESEEESVSL or the 9-residue peptides lacking its N- or C-terminus.