Fig. 4.
Fig. 4. Minigene encoding H-Y epitope is recognized by the HLA-B60 HY CTL. / HeLa cells were transfected with UTY minigene cDNA 79-120, 172-216, or 484-522 together with HLA-B60 cDNA (▧), or with UTY minigene cDNA only (▪). Forty-eight hours after transfection, HLA-B60 HY CTL was added. Culture supernatants were harvested 1 day later and tested for TNF production.

Minigene encoding H-Y epitope is recognized by the HLA-B60 HY CTL.

HeLa cells were transfected with UTY minigene cDNA 79-120, 172-216, or 484-522 together with HLA-B60 cDNA (▧), or with UTY minigene cDNA only (▪). Forty-eight hours after transfection, HLA-B60 HY CTL was added. Culture supernatants were harvested 1 day later and tested for TNF production.

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