Fig. 3.
![Fig. 3. Stimulation of HEL cells with convulxin. / (A) For 3 days, control and PMA-differentiated HEL cells were stimulated with convulxin (20 nmol/L) at different times, lysed, and subjected to SDS-PAGE, then blotted for tyrosine phosphorylation using mAb 4G10. Arrows indicate the major tyrosine phosphorylated bands. (B) Immunoprecipitation of the indicated proteins and tyrosine phosphorylation blot as in panel A. Membranes were reprobed for detection of the corresponding proteins to check equal loading. (C) [Ca++]i increase on convulxin and thrombin stimulation before and after differentiation with PMA. Values indicate increase in [Ca++]i ± SEM. Results are representative of 5 experiments, with 20 cells being measured in each experiment.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/8/10.1182_blood.v96.8.2740/5/h82000250003.jpeg?Expires=1722614037&Signature=vwnIRNAOpxEX9Y1b9bTMoFRAfJPGH4l8bO6N7Cl5Nn7fhczO19KTx1EbUblk0vS1KYsGbpfo69wI15PI7M6h3uFJCxsgbMwsl9HuIRVeMQWxHoxPDETAQNyPbueJoKpQBOXyYbf8lu0S2PrlMh81g6aTBLKM6axB68pG0tdHrCZva3JNefkTkxDRU2AVRlZrKHbPc-51GZOc1n189Vfys1ix45MX-jAggW~~N-Lf6UirgGqcxS3CbzPBQmbqB1Y9ZBQpN1rGwYjahYQptX~wXg-cTazw48XfFVoF973Lwtvr0MtubVq9LH82v64OZ5-hoPziCgsq3yvI2XMGtlsyfA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Stimulation of HEL cells with convulxin.
(A) For 3 days, control and PMA-differentiated HEL cells were stimulated with convulxin (20 nmol/L) at different times, lysed, and subjected to SDS-PAGE, then blotted for tyrosine phosphorylation using mAb 4G10. Arrows indicate the major tyrosine phosphorylated bands. (B) Immunoprecipitation of the indicated proteins and tyrosine phosphorylation blot as in panel A. Membranes were reprobed for detection of the corresponding proteins to check equal loading. (C) [Ca++]i increase on convulxin and thrombin stimulation before and after differentiation with PMA. Values indicate increase in [Ca++]i ± SEM. Results are representative of 5 experiments, with 20 cells being measured in each experiment.
