Fig. 1.
GPVI expression in megakaryoblastic cell lines and platelets.
(A) RNA (2 μg) was reverse transcribed and one fifth of the cDNA obtained used for PCR amplification. RT-PCR products were separated by agarose gels and stained with ethidium bromide to visualize GPVI and β-actin. (B) Fluorescent profile of representative cell lines. Cells were incubated with convulxin and a specific antibody to convulxin, then indirectly stained with FITC-conjugated antirabbit IgG (Fab′)2 fragment (shaded area). The nonshaded areas show background labeling observed in the absence of convulxin. Cytofluorographs were obtained on a FACsort. Results are representative of 5 experiments.
