Desensitizing effect of PBP and CTAP-III on NAP-2–induced PMN degranulation.

(A) Degranulation response of PMN toward increasing concentrations of NAP-2 after pretreatment of cells for 10 minutes with or without 50 nmol/L PBP or CTAP-III. Data from 1 of 3 representative independent experiments with PMN from different donors are shown. (B) Degranulation response of PMN to 40 nmol/L NAP-2 after 10-minute pretreatment with or without increasing concentrations of PBP or CTAP-III. Data are expressed as the percentage of the elastase release response from cells left unexposed to the desensitizing agents, and they represent means ± SD of data obtained in 3 independent experiments. CTAP-III, ●; PBP, ▴; no desensitizing agent, ▪.