The c-fes expression cassette directs myeloid specific expression of a heterologous gene in hematopoietic tissues.

Representative data from a flow cytometry analysis of hematopoietic cells from a transgenic mouse expressing a c-fes EGFP construct (tg) and a nontransgenic littermate (neg). (A) Bone marrow. Of the cells, 33% express the transgene. Approximately 50% of the Gr-1⁺ cells coexpress EGFP. The same is true for the Mac-1⁺. The B-cells (B220⁺) are EGFP⁻. (B) Spleen. Approximately 5% of the cells express the transgene, and expression is observed only in Gr-1⁺ or in Mac-1⁺ cells. (C) Thymus. Expression of EGFP is absent or very low, indicating that the c-fes expression cassette is inactive in T cells. In the double histograms, the numbers indicate percentages of cells present in the given quadrant. Cell-density scaling parameters are identical for all double histograms from the same tissue, but differ slightly between bone marrow and spleen. There were 50 000 counts from each sample analyzed.