Flow cytometric analysis of P-selectin–Ig binding to PSGL-1 on KG1a cells after mocarhagin digestion.

(A) staining pattern of isotype control (human IgG1) antibody, followed by protein A-FITC; (B) buffer-treated cells stained with P-selectin–Ig chimera, followed by protein A-FITC; (C) mocarhagin-treated cells stained with P-selectin–Ig, followed by protein A-FITC. Note shift in fluorescence intensity of P-selectin–Ig binding to PSGL-1 after mocarhagin digestion, compared with buffer-treated cells.