Directed migration of T cells in the absence of a gradient and under flowing conditions.

Cells were plated on an Fn-coated coverslip in contact with Fn alone (A) or overlapping a stripe of SDF-1α coated on the Fn as outlined in the Figure (B). The coverslip was placed in a flow chamber, as described, so that the flow was orthogonal to the direction of migration and was incubated for 2 additional hours. Although the cells in A remained within the boundaries of the original spot, a subset of cells in B clearly demonstrated directed migration to the stripe of SDF-1α.