FISH detection of DOK1.

Metaphase spreads from synchronized human peripheral lymphocytes were hybridized with a digoxigenin-11-dUTP–labeled DOK1 probe. The hybridization signals were detected with rhodamine-conjugated antidigoxigenin antibodies. Normal metaphase spread after FISH (left panel) shows localization of DOK1 on chromosome 12 (arrows). Inverted DAPI banding (right panel) simulates G-banding for chromosomal identification. Inset displays subchromosomal localization of the gene on 12p12.3-13.1.