Skewing of the TCR BV7 repertoire in patients with PNH and identification of the BJ gene segment used in the clonal BV7 T-cell expansion.

Eight PNH patients displayed skewing in the TCR family BV7. In 6 patients further refinement of the TCR family BV7 (left column) repertoire was performed by using FAM-labeled primers, specific for the 13 different BJ gene segments. The BJ segment used in the clonal expansion was identified as a single peak or in the case of patient MSK28, as 2 single peaks (right column).