![Fig. 11. Intracellular Ca++-mobilizing activity of IL-8(1-77) and IL-8(7-77) in CXCR1- and CXCR2-transfected cell lines. / Intracellular Ca++-mobilizing capacities of IL-8(1-77) and IL-8(7-77) were compared on cell lines transfected with CXCR1 and CXCR2. In both cell lines, IL-8(7-77) was more active than IL-8(1-77), but this difference was more pronounced in CXCR1- (A) than in CXCR2- (B) transfected cell lines (P < .05). One representative experiment of 3 is shown. Successive one-third dilutions of both IL-8 forms were analyzed until no increase in [Ca++]i could be measured. Detection limits in [Ca++]i increase were 50 and 20 nmol/L for CXCR1 and CXCR2 transfectants, respectively. For each IL-8 variant and CXCR interaction, dosages giving a response below the detection limit of the [Ca++]i increase were included.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/8/10.1182_blood.v96.8.2673/5/h82000242011.jpeg?Expires=1724242648&Signature=BSQ7tf1sLxyg0RZsEPJdR3dxPN4b0i1A6t68obKJ7ffUmZIh7wbm4iwhxGJ8kWJqfkTk13JllQRr9ugaIKPOySDQ~fIkpKSCI0LAmPb~VvUGeVIjifnOmItbtCmmkKVZKHRbYactZOQc8sKtsiuxOYlz~e1dZji~1Wu42t3f5-HGnrMaCOFumm7oIf1yhADiHiET~D-W7aVraB89t7c6SPYWxkt~oUGOAs0WBP6tjlJEhMkcE1nkONycq06EruDAMQFr2Yy~a5s9mILi4prJQJ8vN3o~ydXv2KYIwGX1nZ8DWL8L55LqEkkUZqsnOIdWjQsMnPXVlJlCJlPx6MKC2A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Intracellular Ca++-mobilizing activity of IL-8(1-77) and IL-8(7-77) in CXCR1- and CXCR2-transfected cell lines.
Intracellular Ca++-mobilizing capacities of IL-8(1-77) and IL-8(7-77) were compared on cell lines transfected with CXCR1 and CXCR2. In both cell lines, IL-8(7-77) was more active than IL-8(1-77), but this difference was more pronounced in CXCR1- (A) than in CXCR2- (B) transfected cell lines (P < .05). One representative experiment of 3 is shown. Successive one-third dilutions of both IL-8 forms were analyzed until no increase in [Ca++]i could be measured. Detection limits in [Ca++]i increase were 50 and 20 nmol/L for CXCR1 and CXCR2 transfectants, respectively. For each IL-8 variant and CXCR interaction, dosages giving a response below the detection limit of the [Ca++]i increase were included.
