Fig. 2.
Investigation of the intracellular stability of wild-type and Val34Leu mutant recombinant FXIII-A by pulse chase analysis.
Metabolic labeling of COS cells transfected with wild-type or mutant FXIII-A was performed by incubating the cells with35S-methionine. The presence of labeled FXIII antigen was analyzed after variable chase periods (shown are the 8-hour and 24-hour periods) by immunoprecipitating the cell lysates with FXIII A-subunit antibody and subjecting the precipitated proteins to 10% SDS-PAGE. Radiolabeled FXIII-A was visualized by autoradiography. Met242Thr represents a mutation associated with instability of the protein and FXIII deficiency.
