Fig. 1.
Fig. 1. Molecular genetic analysis of FXIII Val34Leu polymorphism. / (A) Restriction digestion pattern of PCR products. Mr, 50-bp ladder with a double-intensity band of 250 bp. Because the homozygous mutant (L/L) genotype has no restriction site forCfoI, only the nondigested 114-bp PCR product could be seen. In the V/L heterozygous sample, both the intact product (114 bp) and the 94-bp fragment were present. In the wild-type (V/V) sample, the 94-bp single band demonstrates complete digestion. (B) Evaluation of the PCR–restriction digestion method based on amplification created a restriction site by fluorescent DNA sequencing. Representative electropherograms corresponding to the respective genotype are shown.

Molecular genetic analysis of FXIII Val34Leu polymorphism.

(A) Restriction digestion pattern of PCR products. Mr, 50-bp ladder with a double-intensity band of 250 bp. Because the homozygous mutant (L/L) genotype has no restriction site forCfoI, only the nondigested 114-bp PCR product could be seen. In the V/L heterozygous sample, both the intact product (114 bp) and the 94-bp fragment were present. In the wild-type (V/V) sample, the 94-bp single band demonstrates complete digestion. (B) Evaluation of the PCR–restriction digestion method based on amplification created a restriction site by fluorescent DNA sequencing. Representative electropherograms corresponding to the respective genotype are shown.

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