Decreased cell viability and the induction of apoptosis in PEL cells treated with Bay 11.
BC1, BC2, BC3, and BJAB cells were placed in culture at 7.5 × 105 cells/mL without Bay 11 or with 5 μmol/L Bay 11 for 24 hours. (A), Cell viability was assessed by hemocytometry and trypan blue exclusion, and is reported as the percentage of viable cells remaining at 24 hours, relative to the initial viable cell number (100%). Data shown are compiled from 5 independent experiments, and are shown for both untreated (continuous line) and treated (dashed line) cells. (B), Induction of apoptosis in PEL cells on inhibition of NF-κB. 2 × 105 cells were labeled with FITC-conjugated annexin-V and analyzed by flow cytometry. The percentage of apoptotic cells from 4 independent experiments are reported for both untreated (white bar) and Bay 11 treated (black bar) cells. (C), DAPI staining of BC3 cells with or without treatment with 5 μmol/L Bay 11 for 24 hours shows significant nuclear condensation and fragmentation in the treated cells.
