Fig. 3.
Inhibition of constitutive NF-κB activity in BC3 cells.
BC3 cells placed in culture at 7.5 × 105 cells/mL were treated for 1 hour with the inhibitors SN50 (A), CAPE (B), and Bay 11-7082 (C). Of the inhibitors tested only Bay 11-7082 inhibited NF-κB/DNA binding within 1 hour of treatment. Inhibition observed with Bay 11 was dose dependent, with nearly complete inhibition at the 5 and 10 μmol/L concentrations. Additional studies in which cells were analyzed for up to 24 hours after treatment or were treated with the inhibitors SN50 and CAPE every 2 hours for a 6-hour period yielded the same results.
