Measurement of the oxidative burst response of mature eosinophils.

Eosinophils are derived from either wild-type (▪) or Syk^−/− (░) fetal liver upon stimulation with PMA (20 ng/mL), normal human serum-opsonized zymosan (OPZ, 1 mg/mL), heat-inactivated normal human serum-opsonized zymosan (hiOPZ, 1 mg/mL), and human IgG (plate precoating) as determined by cytochromec reduction. Each bar represents the mean ± SEM of 3 independent experiments.