Changes in expression of the lineage-specific transcription factors during megakaryocyte, granulocyte, or macrophage differentiation in each transfectant.

(A) Expression of PU.1 and c-myb mRNA in 32D/ras/GATA-1 during IPTG treatment. 32D/ras/GATA-1 cells were treated with IPTG for the indicated times and subjected to Northern blot analysis. (B) Expression of GATA-1 mRNA in K562/ras/PU.1 and K562/ras/c-myb during IPTG treatment. K562/ras/PU.1 and K562/ras/c-myb were treated with IPTG, and the expression of GATA-1 mRNA was examined by Northern blot analysis.