Effects of PU.1 and c-Myb on the lineage phenotype of K562.

(A) DNA content analysis before and after 5-day IPTG treatment. DNA content of the cultured cells was examined by propidium iodide staining and analyzed with flow cytometry. (B) Flow cytometric analysis on the surface expression of GPA, GPIIb/IIIa, CD11b, CD14, and CD33 before and after 5-day IPTG treatment. Surface phenotypes of the cultured cells were examined with the indirect immunofluorescence method by staining with the indicated antibody () and control antibody of the same isotype (····).