Enforced expression of PU.1 and c-Myb in K562/ras.

(A, B) Western blot analyses on PU.1 and c-Myb expression. Expression levels of PU.1 and c-Myb were examined in each clone by Western blot analyses on total cellular lysates. (C) Light micrograph of K562/ras/Mock, K562/ras/PU.1, and K562/ras/c-myb before and after 5-day culture with IPTG. Cells were cultured with or without IPTG for 5 days. Cytocentrifugation preparation was stained with May–Grünwald–Giemsa (original magnification, ×100).