Effects of GATA-1 transgene on lineage phenotype of 32D/ras.

(A) Expression of GATA-1 in 32D/ras, 32D/ras/Mock, 32D/ras/GATA-1, and MEL. Total cell lysates were subjected to SDS-PAGE and probed with anti-GATA-1 and anti-actin antibodies. (B) Benzidine staining of the cultured cells. Cells were suspended in benzidine staining solution and subjected to cytospin centrifugation. Black-stained cells were counted as benzidine-positive cells by microscopy. Results are shown as the mean ± SD of triplicate experiments. (C) Flow cytometric analyses on CD61 expression. Expression of CD61 was examined by staining with anti-CD61 antibody () and control antibody of the same isotype (····).