Effect of cell culture density on stem cell expansion and myeloid cell yield.

Unselected PBPC were seeded into cultures at 1 of 3 different cell densities. (A) Fold expansion was calculated by dividing the number of viable myeloid cells obtained after 9 days of culture by the number of CD34⁺ cells at culture initiation. (B) Myeloid cell yield was determined by dividing the number of viable myeloid cells harvested on day 9 by the total number of PBPC seeded into the cultures. Results are reported as the mean ± SD. Statistics were calculated using analysis of variance.