Staining of CAG myeloma cells against CD43, CD38 and syndecan-1.

Evidence of uropod targeting as a dynamic process. CAG cells were stained against CD43 either at 20°C after fixation (A) or at 37° before fixation (B). Uropod restriction of CD43 was seen only when live cells were stained. (C) shows uniform expression of CD38 over the cell surface in cells stained live at 37°C. (D) shows lack of syndecan-1 polarization in cells stained live at 4°C.