Fig. 5.
Fig. 5. Destabilization of p210Bcr-Abl, Raf-1, and tyrosine phosphorylated proteins by HA, radicicol, and KF58333 treatment. / K562 cells were cultured with 0.3 μmol/L of HA, radicicol, and 0.05 μmol/L of KF58333 for indicated times and analyzed by Western blotting as described in “Materials and methods.” Total cell lysates were subjected to Western blotting with antityrosine phosphorylated proteins antibody (A), anti-Abl antibody (B), anti-Raf-1 antibody (C), and anti-Erk antibody (D).

Destabilization of p210Bcr-Abl, Raf-1, and tyrosine phosphorylated proteins by HA, radicicol, and KF58333 treatment.

K562 cells were cultured with 0.3 μmol/L of HA, radicicol, and 0.05 μmol/L of KF58333 for indicated times and analyzed by Western blotting as described in “Materials and methods.” Total cell lysates were subjected to Western blotting with antityrosine phosphorylated proteins antibody (A), anti-Abl antibody (B), anti-Raf-1 antibody (C), and anti-Erk antibody (D).

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