Coexpression of N-CoR potentiates transcriptional repression by TEL, but not by TEL lacking the N-CoR interaction domain.

TEL, TEL(Δ53-116), or TEL(Δ119-336) expression plasmid (50 ng) was cotransfected with 200 ng of a luciferase reporter containing a single ETS consensus binding site upstream of HSV-Tk minimal promoter (TEL-RE-TkLUC) and 100 ng expression vectors for N-CoR and/or mSin3A as indicated. The expression of TEL had no effect on the reporter activity in the absence of the ETS binding site, and there were no significant variations observed in the expression of TEL proteins between different samples (data not shown).