American Society of Hematology

Fig. 4.

$Fig. 4. Cell expansion, transduction efficiency, and yield of transduced cells. / Cell expansion (top), transduction efficiency (middle), and yield of transduced cells (bottom) were measured after stimulation of CD34+ BM cells for 3 days in serum-free medium supplemented with FL + SF + IL-3 + HIL-6 and then exposure to VCM once for a total of 48 hours or 3 times over a total period of 96 hours. To reduce the variation of the data due to differences in absolute transduction efficiencies between different experiments, the results for the second protocol have been expressed as a fraction of the corresponding result obtained with the first protocol in the same experiment. These values have then been pooled and the mean ± SEM shown (n = 3).$

Cell expansion, transduction efficiency, and yield of transduced cells.

Cell expansion (top), transduction efficiency (middle), and yield of transduced cells (bottom) were measured after stimulation of CD34⁺ BM cells for 3 days in serum-free medium supplemented with FL + SF + IL-3 + HIL-6 and then exposure to VCM once for a total of 48 hours or 3 times over a total period of 96 hours. To reduce the variation of the data due to differences in absolute transduction efficiencies between different experiments, the results for the second protocol have been expressed as a fraction of the corresponding result obtained with the first protocol in the same experiment. These values have then been pooled and the mean ± SEM shown (n = 3).

This Feature Is Available To Subscribers Only