Time-course study of changes in pit-1 expression in cultures of CD34⁺CD38⁻ BM cells stimulated with FL + SF + IL-3 + H-IL-6.

RNA obtained from cells immediately after their purification and then 1, 3, and 5 days later was reverse transcribed and amplified by polymerase chain reaction as described in Materials and methods, and the resultant cDNA electrophoresed, blotted, and hybridized first with a pit-1 probe and then a GAPDH probe. The results of 3 independent experiments are shown in the top 2, middle 2, and bottom 2 rows of autoradiographs, respectively.