NK cytotoxic activity in the progeny of CD7⁺CD34⁻Lin⁻ SP cells.

Panels A and B depict 7AAD uptake by CFSE-labeled K562 cells when cultured 4 hours without effectors (A) as compared with those cultured 4 hours with putative NK cells generated from CD7⁺CD34⁻Lin⁻ SP under condition D (B). Panel C depicts the percentage of cell lysis for K562 or Raji target cells at various effector-to-target ratios. The effector cells were putative NK cells derived from CD7⁺CD34⁻Lin⁻ SP cells grown under condition D (Table 1). These data are representative of 2 cultures (of 3 tested) where lysis of K562 target cells was clear.