Western blot of cell lysates incubated with monoclonal antibody to the FcεRI α chain.

For this experiment, 5.5 × 10⁶ basophils or AML14-3D10 cells were lysed, and 500 000 cells per lane were loaded on a gel, electrophoresed, and assessed by Western blot analysis. Basophils have a band of approximately 50 kd that corresponds to FcεRIα; this is absent in the AML14-3D10 cells. Results shown are representative of 3 experiments.