IL-8 production is dependent on protein synthesis.

Eosinophils were incubated in the presence of GM-CSF and cycloheximide (1 μmol/L; open bars) or GM-CSF alone (closed bars). After 30 minutes, cells were transferred to wells containing the stimuli indicated on the x-axis. Twenty-four hours later, the IL-8 content of the supernatants was determined by ELISA (3 experiments).