American Society of Hematology

Fig. 5.

$Fig. 5. Colocalization of SNAP-23 and syntaxin 6 in activated human neutrophils (PMNs) by confocal laser scanning microscopy. / Resting and PMA-activated human neutrophils were permeabilized and processed for double-labeled immunofluorescence with anti–SNAP-23 and anti–syntaxin 6 antibodies. The immuno-decorated areas were detected with the appropriate secondary antibodies coupled to phycoerythrin (red fluorescence for anti-SNAP-23) or FITC (green fluorescence for anti–syntaxin 6) by using confocal laser scanning microscopy, as described in “Materials and methods.” Overlay of the adjacent panels (right panels) shows colocalization of SNAP-23 and syntaxin 6 at the cell surface of activated human neutrophils. Areas of colocalization are yellow. Bar, 3 μm.$

Colocalization of SNAP-23 and syntaxin 6 in activated human neutrophils (PMNs) by confocal laser scanning microscopy.

Resting and PMA-activated human neutrophils were permeabilized and processed for double-labeled immunofluorescence with anti–SNAP-23 and anti–syntaxin 6 antibodies. The immuno-decorated areas were detected with the appropriate secondary antibodies coupled to phycoerythrin (red fluorescence for anti-SNAP-23) or FITC (green fluorescence for anti–syntaxin 6) by using confocal laser scanning microscopy, as described in “Materials and methods.” Overlay of the adjacent panels (right panels) shows colocalization of SNAP-23 and syntaxin 6 at the cell surface of activated human neutrophils. Areas of colocalization are yellow. Bar, 3 μm.

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