American Society of Hematology

Fig. 3.

$Fig. 3. Subcellular localization of SNAP-23 and syntaxin 6 in resting and activated human neutrophils. / (A,C) Localization of SNAP-23 and syntaxin 6 in the membrane fraction. Equal amounts of postnuclear extract (E), soluble (S), and membrane (M) proteins (60 μg), prepared from resting human neutrophils as described in “Materials and methods,” were run on SDS-polyacrylamide gels and analyzed by immunoblotting, using anti–SNAP-23 polyclonal antibody (A) or 3D10 anti–syntaxin 6 MoAb (C). (B,D) Subcellular distribution of SNAP-23 and syntaxin 6 in resting and PMA-activated human neutrophils (PMNs). Equal amounts of total protein (30 μg), prepared from the subcellular fractions 2-8 from resting and activated human neutrophils prepared as described in “Materials and methods,” were run on SDS-polyacrilamide gels and analyzed by immunoblotting, using anti–SNAP-23 polyclonal antibody (B) or 3D10 anti–syntaxin 6 MoAb (D). Fractions enriched in plasma membrane (PM), tertiary granules (TG), specific granules (SG), and azurophilic granules (AG) were analyzed. The molecular masses (kd) of protein markers are indicated on the left.$

Subcellular localization of SNAP-23 and syntaxin 6 in resting and activated human neutrophils.

(A,C) Localization of SNAP-23 and syntaxin 6 in the membrane fraction. Equal amounts of postnuclear extract (E), soluble (S), and membrane (M) proteins (60 μg), prepared from resting human neutrophils as described in “Materials and methods,” were run on SDS-polyacrylamide gels and analyzed by immunoblotting, using anti–SNAP-23 polyclonal antibody (A) or 3D10 anti–syntaxin 6 MoAb (C). (B,D) Subcellular distribution of SNAP-23 and syntaxin 6 in resting and PMA-activated human neutrophils (PMNs). Equal amounts of total protein (30 μg), prepared from the subcellular fractions 2-8 from resting and activated human neutrophils prepared as described in “Materials and methods,” were run on SDS-polyacrilamide gels and analyzed by immunoblotting, using anti–SNAP-23 polyclonal antibody (B) or 3D10 anti–syntaxin 6 MoAb (D). Fractions enriched in plasma membrane (PM), tertiary granules (TG), specific granules (SG), and azurophilic granules (AG) were analyzed. The molecular masses (kd) of protein markers are indicated on the left.

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