Generation of GST–SNAP-23 fusion protein and characterization of anti–SNAP-23 antibody.

(A) Bacterial extract and purified GST–SNAP-23 fusion protein were run in SDS-polyacrylamide gel electrophoresis and stained with Coomassie Blue. (B) Characterization of the specificity of the polyclonal rabbit antibody against SNAP-23. Different amounts of purified recombinant human SNAP-23 and rat SNAP-25 proteins were electrophoresed and blotted with anti-SNAP-23 antibody. Bands were visualized by using an enhanced chemiluminescence system. The molecular masses (kd) of protein markers are indicated on the left.