Quantitative competitive PCR analysis of p190 wild-type and p190 del C expression.
(A) RT-PCR analysis of wild-type p190 expression. Primers are as indicated beneath the lanes. Lanes 1, 3, 7, and 9, p190 wild-type no. 1; lanes 2, 4, 8, and 10, p190 wild-type no. 2. Lanes 1, 2, 7, and 8, bone marrow; lanes 3, 4, 9, and 10, peripheral blood. Lanes 5 and 11, negative control (no RNA added); lanes 6 and 12, positive control no. 2207 RNA. m = φX/HaeIII marker. (B) Quantitative competitive PCR analysis of p190 wild-type and del C expression. Arrows point to the location of the transgenic BCR/ABL 305-bp and the 260-bp competitor fragments. The reactions shown in lanes 1 to 6 (p190 wild-type no. 1) and lanes 12 to 14 (p190 del C animal no. 4445; no. 4208 line) each contained 2 μg of total bone marrow RNA and varying amounts of competitor: 0.5 × 107 molecules, lane 1; 0.5 × 106, lane 2; 0.5 × 105, lanes 3 and 12; 0.5 × 104, lanes 4 and 13; 0.5 × 103, lanes 5 and 14; no competitor, lane 6; positive control RNA no. 2207, lane 7; no RNA, lanes 8 and 11; and 104 and 105 molecules competitor only, lanes 9 and 10, respectively.
