Expression of the Bcr/Abl p190 del C mutant.

(A) Western blot analysis of expression of p190 Bcr/Abl proteins in COS cells. Lanes 1 and 2 were reacted with antiserum directed against the C-terminal end of Abl (Oncogene Science, Inc, c-Abl Ab-2), whereas lanes 3 and 4 were incubated with antibodies directed against more upstream sequences of Abl (Oncogene Science, Inc, c-Abl Ab-3). COS-1 cells were transfected with p190 wild type (lanes 1,3) or p190 del C (lanes 2,4). (B) Schematic representation of the Bcr/Abl p190 del C mutant protein. The dotted region represents the N-terminal oligomerization domain of Bcr; the open box, other sequences encoded by Bcr exon 1. The hatched area represents the Abl protein moiety.