Exclusive myeloid repopulation by 5q-deleted NOD/SCID reconstituting stem cells.

FACS profile shows BM from 4 pooled NOD/SCID mice each having transplantation with 700 000 CD34⁺ cells isolated from MDS patient no. 12. (A) BM cells were stained with antimouse CD45.1-APC (Ly 5.1) and antihuman CD45-FITC. Human CD45⁺ cells constituted 12% of the whole mouse BM. (B) Coexpression of CD15 and CD33 on human CD45⁺ cells. BM was gated on huCD45⁺ cells and investigated for the expression of huCD15 and huCD33. Note that virtually all cells expressed myeloid markers, as confirmed by the absence of CD19 expression (data not shown).