FISH analysis of CD34⁺CD38⁻ candidate stem cells from a patient with 5q− syndrome.

BM CD34⁺CD38⁻ cells were isolated from a normal donor (A) and patient no. 1 with 5q− syndrome (B) and subjected to FISH analysis as described in “Materials and methods.” The nuclei of normal cells appear as 2 green (5p) and 2 orange (5q), whereas 5q-deleted cells have 2 green signals and 1 orange signal.