Analysis of V_H constant region transcripts from case 5334.

L_HJ_H sequences are shown for reference from FWR3. IgM, IgD, IgG1, and IgA transcripts were detected using a CDR3-specific primer together with constant region primers. IgM and IgD sequences were virtually identical. IgG1 transcripts differed by a further mutation in codon 100c. Some IgA transcripts appeared to be related to the IgM/D group, whereas others had acquired the additional mutation in codon 100c, which was observed in the IgG1 group. All sequences are shown for the constant region transcripts. In total, 43 tumor-related sequences were analyzed.