Fig. 1.
Fig. 1. A 36- to 38-kd protein is tyrosine phosphorylated on either CD2 or CD3 stimulation of Jurkat T cells. / Wild-type Jurkat T cells were either left unstimulated, stimulated with the mitogenic pair of anti-CD2 mAbs T112 and T113, or stimulated with anti-CD3ε mAb OKT3 at 37°C for 5 minutes. Cells were then lysed in 1% Brij97 lysis buffer, and clarified PNLs (0.3 × 106 cell equivalents) were resolved on SDS-PAGE, transferred to PVDF membranes, and immunoblotted with the antiphosphotyrosine antibody 4G10. Tyrosine phosphorylation of a 36- to 38-kd protein is shown at the arrow.

A 36- to 38-kd protein is tyrosine phosphorylated on either CD2 or CD3 stimulation of Jurkat T cells.

Wild-type Jurkat T cells were either left unstimulated, stimulated with the mitogenic pair of anti-CD2 mAbs T112 and T113, or stimulated with anti-CD3ε mAb OKT3 at 37°C for 5 minutes. Cells were then lysed in 1% Brij97 lysis buffer, and clarified PNLs (0.3 × 106 cell equivalents) were resolved on SDS-PAGE, transferred to PVDF membranes, and immunoblotted with the antiphosphotyrosine antibody 4G10. Tyrosine phosphorylation of a 36- to 38-kd protein is shown at the arrow.

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