Fig. 4.
![Fig. 4. Sickle mononuclear leukocytes activate NF-κB in MVEC. / Mononuclear leukocytes (15 leukocytes/endothelial cell) or TNF-α (10 ng/mL) were incubated with MVEC for 1 hour. After incubation, MVEC nuclear proteins were extracted for NF-κB analysis. Nuclear extracts (10 μg protein) were incubated with 2 ng [32P]-labeled dsDNA containing a consensus NF-κB sequence and analyzed by electrophoretic mobility shift assay. Lane 1, [32P]-labeled dsDNA probe alone; lane 2, control (media only)-treated MVEC; lane 3, TNF-α–treated MVEC; lane 4, normal mononuclear leukocyte-treated MVEC; lane 5, sickle mononuclear leukocyte-treated MVEC. Results are from a single representative experiment of 2.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/7/10.1182_blood.v96.7.2451/5/h81900196004.jpeg?Expires=1723536928&Signature=OJFWQyq-ZwE5hDbWRB~MbFMjQEWApnTG0zMESk-eVzz~ZxjXtEh8VosKrqDO~M2QBwSDoWJIa~1dxXJEvCEhThAtjmMmmW0ZCgo1RMtnXuoZhf2JTmTbL37IbR0cCqr70HFn5uEr7sVstz7SO5175vZgCnGK920v0GYYbIHhTa~eWV-L7ZO43yXZ1JGKSODnKOHr8l5QwpBk3gXFvMFlwDgjWko0VNoRRnbWKoS2TD8X1Fd0Zbzd-Q6Ho7Gp7KjT5gzuQ8DaFq4zdeRP6QQIMPuEZfTYoo97-ZsoFLE2Hikefvlji0WQaAqx7Xf1okp4Azxomfgf10HUERhNX8KNqg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Sickle mononuclear leukocytes activate NF-κB in MVEC.
Mononuclear leukocytes (15 leukocytes/endothelial cell) or TNF-α (10 ng/mL) were incubated with MVEC for 1 hour. After incubation, MVEC nuclear proteins were extracted for NF-κB analysis. Nuclear extracts (10 μg protein) were incubated with 2 ng [32P]-labeled dsDNA containing a consensus NF-κB sequence and analyzed by electrophoretic mobility shift assay. Lane 1, [32P]-labeled dsDNA probe alone; lane 2, control (media only)-treated MVEC; lane 3, TNF-α–treated MVEC; lane 4, normal mononuclear leukocyte-treated MVEC; lane 5, sickle mononuclear leukocyte-treated MVEC. Results are from a single representative experiment of 2.
