Northern blot analysis of CCR3 mRNA.

Total RNA (4 μg) from the eosinophils (eos) of 2 individuals and from butyric acid/IL-5 differentiated AML14.3D10 cells (dAML) was hybridized with a radiolabeled full-length CCR3 ORF probe under high stringency conditions. Autoradiography was performed for 72 hours. The locations of 28 S and 18 S RNA are shown on the right and hybridized bands are labeled with an arrowhead.