Fig. 6.
Bcl-XL expression is mediated by cooperation between STAT5 and PI3K.
Ba/F3 cells inducibly expressing STAT5-1*6 were incubated in RPMI 1640 10% FCS, suplemented with 10% WEHI supernatant as a source of IL-3. The cells were either left untreated or induced with doxycycline for 8 hours before additional drug treatment. Doxycycline-induced cells were treated with DMSO (0.05 % v/v), PD98059 (20 μmol/L), LY 294002 (25 μmol/L), or rapamycin (5 nmol/L) for 18 hours. The cells were then processed as described above for immunoblot analysis with the indicated antibodies. Equal loading is verified by p85-PI3K blot.
