Fig. 5.
Fig. 5. Expression of a dominant negative STAT5 mutant inhibits Bcl-XL expression. / Ton.p210.ΔSTAT5 cells were kept in culture in RPMI 1640 + 10% FCS and left untreated or treated with 1 μg/mL for 48 hours. Cells were then harvested, washed, and lysed. Lysates were separated by SDS-PAGE and the level of Bcl-XL was assessed by Western blot. The equal loading is assessed either by the endogenous level of STAT5 (upper panel, STAT5) or by the level of expression of p85 (middle panel).

Expression of a dominant negative STAT5 mutant inhibits Bcl-XL expression.

Ton.p210.ΔSTAT5 cells were kept in culture in RPMI 1640 + 10% FCS and left untreated or treated with 1 μg/mL for 48 hours. Cells were then harvested, washed, and lysed. Lysates were separated by SDS-PAGE and the level of Bcl-XL was assessed by Western blot. The equal loading is assessed either by the endogenous level of STAT5 (upper panel, STAT5) or by the level of expression of p85 (middle panel).

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal