Bcr/Abl increases

Bcl-X gene transcription through STAT5. (A) Scheme representing the various constructs containing fragments of different length of the Bcl-X gene promoter fused to a luciferase gene reporter construct. Ton.B.210 (B) Ton.B.1*6 (C) or Ton.p210.Δ.STAT5 (D) cells were cotransfected with the indicated constructs and a pCMV βGal plasmid. The cells were resuspended in RPMI 1640 + 10% FCS and left untreated or treated with doxycycline for 24 hours. The reporter gene activity was measured as described in “Materials and methods.” Insert. In parallel to the luciferase assay, the specific transcripts for Bcl-X_L were estimated by semi quantitative PCR as described in “Materials and methods.”