Down-regulation of the endogenous MgcRacGAP expression along with differentiation of HL-60 cells and M1 cells into macrophages.

Poly(A)⁺ RNA was harvested from HL-60 (A) or M1 (B) cells before and after TPA (A) or IL-6 (B) stimulation, respectively, at the indicated time points. Poly(A)⁺ RNA (2 μg) from each condition was subjected to the Northern blot analysis, hybridizing with a human (A) or a mouse (B) MgcRacGAP cDNA probe. A GAPDH cDNA was used as a control probe.