Fig. 1.
Fig. 1. Standard curve for quantitative PCR assays for HHV8 ORF26 gene. / Shown are the Ct values from 4 assays at which a given input of HHV8 DNA-containing plasmid was detected by PCR. Plasmid concentrations tested ranged from 1 to 7.5 × 106 copies per reaction mixture. A linear amplification was obtained for values between 30 and 7.5 × 106 copies. The Spearman rank coefficient of correlation (R) is given.

Standard curve for quantitative PCR assays for HHV8 ORF26 gene.

Shown are the Ct values from 4 assays at which a given input of HHV8 DNA-containing plasmid was detected by PCR. Plasmid concentrations tested ranged from 1 to 7.5 × 106 copies per reaction mixture. A linear amplification was obtained for values between 30 and 7.5 × 106 copies. The Spearman rank coefficient of correlation (R) is given.

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