American Society of Hematology

Fig. 7.

$Fig. 7. Kinetics of splenic DC differentiation and phenotypic variations of CD8α− DCs during reconstitution after transfer of BM cells. / BM cells (2 × 106) from C57 BL/Ka Ly 5.2 donor mice were injected intravenously into γ-irradiated (7 Gy) C57 BL/6 Ly 5.1 Pep3b recipient mice. At the indicated times, mice were analyzed for donor-derived DCs in splenic DC-enriched 1.061-density fractions. The percentage of Ly5.2− and Ly5.2+CD11c+ DCs (contour plots), as well as the percentage of CD8α− and CD8α+ DCs among Ly5.2+ DCs (black histograms) are indicated. Grey profiles show the expression of CD4 and F4/80 by Ly5.2+CD8α− DCs; the percentage of CD4+ or F4/80+ cells is indicated. White profiles represent control stainings. These results are representative of three independent experiments with similar results.$

Kinetics of splenic DC differentiation and phenotypic variations of CD8α⁻ DCs during reconstitution after transfer of BM cells.

BM cells (2 × 10⁶) from C57 BL/Ka Ly 5.2 donor mice were injected intravenously into γ-irradiated (7 Gy) C57 BL/6 Ly 5.1 Pep^3b recipient mice. At the indicated times, mice were analyzed for donor-derived DCs in splenic DC-enriched 1.061-density fractions. The percentage of Ly5.2⁻ and Ly5.2⁺CD11c⁺ DCs (contour plots), as well as the percentage of CD8α⁻ and CD8α⁺ DCs among Ly5.2⁺ DCs (black histograms) are indicated. Grey profiles show the expression of CD4 and F4/80 by Ly5.2⁺CD8α⁻ DCs; the percentage of CD4⁺ or F4/80⁺ cells is indicated. White profiles represent control stainings. These results are representative of three independent experiments with similar results.

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